Top 40 Hits of September 2000
| 1 | Madonna | Music |
| 2 | Modjo | Lady (Hear Me Tonight) |
| 3 | Spiller | Groovejet (If This Ain't Love) |
| 4 | A1 | Take On Me |
| 5 | Robbie Williams | Rock DJ |
| 6 | Sonique | Sky |
| 7 | True Steppers & Dane Bowers featuring Victoria Beckham | Out Of Your Mind |
| 8 | Element Four | Big Brother UK TV Theme |
| 9 | Britney Spears | Lucky |
| 10 | Melanie C | I Turn To You |
| 11 | Bomfunk MC's | Freestyler |
| 12 | Wyclef Jean | It Doesn't Matter |
| 13 | Kylie Minogue | On A Night Like This |
| 14 | Craig David | 7 Days |
| 15 | Mariah Carey & Westlife | Against All Odds |
| 16 | Storm | Time To Burn |
| 17 | Eminem | The Real Slim Shady |
| 18 | S Club 7 | Natural |
| 19 | Janet Jackson | Doesn't Really Matter |
| 20 | Zombie Nation | Kernkraft 400 |
| 21 | Ronan Keating | Life Is A Rollercoaster |
| 22 | Planet Perfecto | Bullet In The Gun 2000 |
| 23 | Sugababes | Overload |
| 24 | Aurora featuring Naimee Coleman | Ordinary World |
| 25 | Richard Blackwood | 1-2-3-4 Get With The Wicked |
| 26 | Tru Faith & Dub Conspiracy | Freak Like Me |
| 27 | 'N Sync | It's Gonna Be Me |
| 28 | Vanessa Amorosi | Absolutely Everybody |
| 29 | Baby D | Let Me Be Your Fantasy |
| 30 | Limp Bizkit | Take A Look Around (Theme From M:I 2) |
| 31 | The Bloodhound Gang | The Ballad Of Chasey Lain |
| 32 | Five & Queen | We Will Rock You |
| 33 | Bon Jovi | Say It Isn't So |
| 34 | Robbie Rivera Presents Rhythm Bangers | Bang |
| 35 | Daphne & Celeste | School's Out |
| 36 | Santana featuring The Product G&B | Maria Maria |
| 37 | Lolly | Girls Just Wanna Have Fun |
| 38 | Destiny's Child | Jumpin' Jumpin' |
| 39 | Darude | Sandstorm |
| 40 | Lene Marlin | Unforgivable Sinner |
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